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Laboratory Processing Facilities

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RUCDR Infinite Biologics has developed a robust and diverse automation infrastructure which serves all of the processing laboratories and a majority of their workflows. The RUCDR automation resource is currently comprised of 18 separate robotic platforms that can operate independently or in concert, depending on the application. Most instruments are equipped with in-line plate storage accessories (plate/tip/rack hotels, Cytomat, etc.) and analytical instrumentation (DTX fluorescent plate reader, barcode scanners, etc.) used for sample tracking and quality control measures during nucleic acid extraction, sample transfer and distribution processes. All instruments are configured with custom labware capable of handling all tube, plate and rack formats utilized in RUCDR or requested by clients for biologics distributions. RUCDR has dedicated technical expertise on all platforms to allow robotics integration across most RUCDR laboratory workflows.

Cell Labs

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RUCDR Infinite Biologics Cell Processing Lab is staffed 24/7/365 by over 40 highly trained technicians and is fully equipped with 6 biosafety level II laboratories with 28 Biosafety cabinets, 62 incubators, and a walk-in cold room. To ensure a zero risk of contamination, all externally produced cell lines are processed and cultured in a separate quarantine lab until they are determined to be free of contamination.

QC Labs

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Our quality control provision is state of the art and in place to ensure sample quality and integrity for downstream analysis.

  • Fluidigm BioMark™ (RUID™ and RUIDGx™ Panel Functional QC) – A rapid high throughput and cost-effective approach for assessing both DNA and RNA quality while capturing critical information on sample performance as well as identity validation via gender and ethnicity measurements. It utilizes validated patent pending panels validated for the Fluidigm Biomark microfluidic dynamic array platform. Custom applications and assay development is also available for project specific needs.
  • Trinean DropSense (Analytical QC w/cDROP Analysis) – High throughput, fully automated cuvetteless spectroscopy utilizing cDROP analytical software which specifically quantitates and provides high quality spectral data (based on UV/VIS spectrum) for QC purposes on a variety of molecules, e.g. DNA, RNA, proteins, oligonucleotides and PCR products. Accurate concentration data is provided with specific contaminant profiles for every sample.
  • BioMicrolab VolumeCheck (Analytical QC) – Utilizes ultrasonic technology to measure sample volume in a 96 well/tube automated format for QC purposes. It provides a cost-effective, non-contact, high throughput alternative to manual QC of sample volumes minimizing opportunities for cross contamination of samples.
  • Caliper LabChipGx (Analytical QC) – One of the components of RNA analytical QC is the assessment of ribosomal subunits using the Caliper Lab-on-chip technology. This high throughput measurement provides a RIN (RNA Integrity Number) score that can be used as a preliminary assessment of RNA quality.

Nucleic Acid Labs

Extensive Lab capabilities

RUCDR Infinite Biologics has developed a robust and diverse automation infrastructure which serves all of the processing laboratories and a majority of their workflows.  RUCDR automation resource is currently comprised of 18 separate robotic platforms that can operate independently or in concert, depending on the application. Most instruments are equipped with in-line plate storage accessories (plate/tip/rack hotels, Cytomat, etc.) and analytical instrumentation (DTX fluorescent plate reader, barcode scanners, etc.) used for sample tracking and quality control measures during nucleic acid extraction, sample transfer and distribution processes. All instruments are configured with custom labware capable of handling all tube, plate and rack formats utilized in RUCDR or requested by clients for biologics distributions. RUCDR has dedicated technical expertise on all platforms to allow robotics integration across most RUCDR laboratory workflows. RUCDR Infinite Biologics utilizes the most advanced automated methods to extract DNA and RNA from various biological sources.

Our Nucleic Acid labs house instrumentation such as QiaSymphony, Hamilton Chemagic Star, and Qiagen Autopure LS. Much of the instrumentation in the Nucleic Acid Labs is redundant in order to eliminate down time due to instrument service. RUCDR strives to be on the forefront of high throughput liquid and sample handling. To meet these ends, we house the most advance automated liquid handling instrumentation including Hamilton MicroLab Star, Hamilton Easy blood Fractionator, Tecan Freedom Evo, and Beckman Coulter BioMek FX.

  • ChemagicSTAR – A flexible liquid handling platform used with a wide range of samples and supports volumes from 50 µl to 10 ml for fully integrated bead-based nucleic acid extraction.
  • Qiagen Autopure - Automated purification of genomic DNA from large-volume blood and cell pellet samples using proven Puregene® chemistry. For increased convenience and flexibility, samples can be processed in batches of 8 or 16. Yields average of 350 µg DNA per 10 ml blood in healthy subjects.
  • Qiagen Universal – A fully automated system with medium- to high-throughput applications in 96-well formats. Supports purification of DNA/RNA from cells, solid tissues, blood, swabs, plasmids and forensic samples using solid phase extraction chemistries.
  • Qiagen QIAsymphony – Enables sample preparation of DNA, RNA, bacterial and viral nucleic acids from a wide range of starting materials in CLIA-certified environment. Moderate to high throughput processing (1-96 samples - in batches of 24) - with sample volumes up to 1 ml using Qiagen’s bead based chemistries.
  • Qiagen QIAcube – Enables fully automated, seamless integration of automated, low-throughput sample purification up to 12 samples using Qiagen’s bead based chemistries.
  • Qiagen EZ1 – Automated and rapid small scale purification of nucleic acids from clinical or forensic samples.
  • Tecan Freedom EVO(s) – Dedicated precision liquid handling used for sample preparation protocols for sequencing, array and QPCR downstream processing.
  • Caliper Sciclone(s) – Fully integrated automation for NextGen sequencing library preparation, sequence capture and normalization directly from primary samples.
  • Hamilton Chemagic STAR(s) – Bead-based extraction and aliquoting integrated platform utilizing Chemagen bead-based extraction chemistries for a variety of tissue types and primary sample volumes. In-line primary sample registration with complete end product aliquot sample management and QC preparation.
  • Hamilton EasyBlood STAR(s) – Completely automated blood fractionation system combining precision pipetting, digital blood fractionation documentation and state-of-the-art aliquoting protocols for the precise and rapid fractionation of serum, plasma, buffy coat and red cell pellet directly from blood collection tubes.
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145 Bevier Road Piscataway NJ 08854-8009
p 732 445 1498 f 732 445 1149 www.rucdr.org