Sample Analysis and Processing

Next-Generation Sequencing Services

Next-Generation Sequencing Services

RUCDR Infinite Biologics provides massively parallel “next-generation” sequencing services on the Ion Torrent, Illumina MiSeq and HiSeq 2000 platforms. Comprehensive service bundles include nucleic acid extraction, integrated sample QC, and fully automated construction of sequencing libraries.

  • Capabilities
    • RNA-seq, Exome-seq and targeted amplicon sequencing applications
    • Automated DNA and RNA extraction for a variety of primary sample types (tissue, whole blood, FFPE, cells)
    • Integrated sample and data QC
    • Whole-transcriptome amplification (WTA) services for limited amounts of precious starting RNA
    • Automated construction of sequencing libraries for DNA-seq and RNA-seq applications. Library indexing strategies enable high order sample multiplexing and flexible adjustment of sequence coverage depth.
    • Multiple tiers of Bioinformatics support available - delivery of unaligned, sample-specific sequence reads, read quality assessments, mapped reads, sample mapping summaries, variant reports, and differential expression analyses
  • Technologies
    • A variety of chemistry options are available, including NuGEN sample and library preparation kits, Illumina TruSeq DNA/RNA and custom amplicon kits, and Agilent SureSelect.
    • NuGEN Mondrian and Caliper SciClone systems for fully automated construction of sequencing libraries.
    • Ion Torrent Semiconductor sequencing on the Ion TorrentTM Personal Genome Machine® (PGM). 314, 316 and 318 chips produce up to 10Mb, 100Mb and 1Gb of raw sequence data in less than 5 hours
    • Illumina MiSeq Personal Sequencer produces 8.5 Gb of high-quality sequence data in less than 40 hours
    • Illumina HiSeqTM 2000 provides industry leading sequencing throughput (600Gb in a single run)

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